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</div><div class="pct"><style type="text/css">.pcb{margin-right:0}</style><div class="pcb"> <div class="t_fsz"> <table cellspacing="0" cellpadding="0"><tr><td class="t_f" id="postmessage_514381"> <a href="http://www.lcgyjg.com/" target="_blank"><font color="#000000">激光</font></a><font color="#000000">扫描共聚焦显微镜(laser scanning confocal microscope LSCM)是20世纪80年代发展起来的一项具有划时代意义的高科技新<a href="http://b.oecr.com/sell" target="_blank" class="relatedlink">产品</a>，是当今世界最先进的细胞生物学分析仪器。它是在荧光显微镜成象的基础上加装<a href="http://laser.oecr.com" target="_blank" class="relatedlink">激光</a>扫描装置，使用紫外光或可见光激发荧光探针，利用计算机进行图象处理，对生物样品进行定性、定量、定时和定位研究。已广泛应用于细胞生物学、生理学、病理学、解剖学、胚胎学、免疫学和神经生物学等领域。</font><br /> <font color="#000000">一. 组成<a href="http://www.sdifk.com/" target="_blank"><font color="#f6e9ce"><font face="宋体"><strong>电动轿车配件</strong></font></font></a><a href="http://www.lcgyjg.com/" target="_blank"><font color="#f6e9ce"><font face="宋体"><strong>激光刻章机</strong></font></font></a><a href="http://www.gangguanhb.com/" target="_blank"><font color="#f6e9ce"><font face="宋体"><strong>无缝管</strong></font></font></a><a href="http://www.sdifk.com/" target="_blank"><font color="#f6e9ce"><font face="宋体"><strong>钨绞丝</strong></font></font></a><a href="http://www.252000.com/" target="_blank"><font color="#f6e9ce"><font face="宋体"><strong>聊城网络公司</strong></font></font></a><a href="http://www.lcgyjg.com/" target="_blank"><font color="#f6e9ce"><font face="宋体"><strong>激光雕刻机</strong></font></font></a></font><br /> <font color="#000000">倒置或直立荧光显微镜、扫描头(<a href="http://light.oecr.com" target="_blank" class="relatedlink">照明</a>针孔、探测针孔、荧光滤片系统、镜扫描系统和光电倍增管)、扫描头控制<a href="http://electronic.oecr.com" target="_blank" class="relatedlink">电路</a>、计算机和图像输出设备</font><br /> <br /> <font color="#000000">二. 原理</font><br /> <font color="#000000">Confocal</font><br /> <font color="#000000">利用放置在光源后的照明针孔和放置在检测器前的探测针孔实现点照明和点探测，来自光源的光通过照明针孔发射出的光聚焦在样品焦平面的某个点上，该点所发射的荧光成像在探测针孔上，该点以外的任何发射光均被探测针孔阻挡。照明针孔与探测针孔对被照射点或被探测点来说是共轭的，因此被探测点即共焦点，被探测点所在的平面即共焦平面。计算机以像点的方式将被探测点<a href="http://display.oecr.com" target="_blank" class="relatedlink">显示</a>在计算机屏幕上，为了产生一幅完整的图像，由光路中的扫描系统在样品焦平面上扫描，从而产生一幅完整的共焦图像。只要载物台沿着Z轴上下移动，将样品新的一个层面移动到共焦平面上，样品的新层面又成像在显示器上，随着Z轴的不断移动，就可得到样品不同层面连续的光切图像。</font><br /> <br /> <font color="#000000">三. 激光扫描共焦显微镜的设计特点:</font><br /> <font color="#000000">1.点照明</font><br /> <font color="#000000">2.具有照明pinhole和探测pinhole</font><br /> <font color="#000000">3.照明pinhole和探测pinhole共轭(共焦), 共焦点即被探测点，被探测点所在的平面为共焦平面</font><br /> <font color="#000000">4.具有扫描系统―― 逐点扫描成像</font><br /> <font color="#000000">5.具有多个（四个） 荧光通道，可同时探测多个被标记物</font><br /> <font color="#000000">例如: Leica TCS NT 的技术指标:<font style="font-size:9pt"><a href="http://www.sdifk.com/" target="_blank"><font color="#f6e9ce"><font face="宋体"><strong>电动轿车配件</strong></font></font></a><a href="http://www.lcgyjg.com/" target="_blank"><font color="#f6e9ce"><font face="宋体"><strong>激光刻章机</strong></font></font></a><a href="http://www.gangguanhb.com/" target="_blank"><font color="#f6e9ce"><font face="宋体"><strong>无缝管</strong></font></font></a><a href="http://www.sdifk.com/" target="_blank"><font color="#f6e9ce"><font face="宋体"><strong>钨绞丝</strong></font></font></a><a href="http://www.252000.com/" target="_blank"><font color="#f6e9ce"><font face="宋体"><strong>聊城网络公司</strong></font></font></a><a href="http://www.lcgyjg.com/" target="_blank"><font color="#f6e9ce"><font face="宋体"><strong>激光雕刻机</strong></font></font></a></font></font><br /> <font color="#000000"><font style="font-size:9pt">a</font></font><font color="#000000">1. xy分辨率比传统显微镜小1.4x</font><br /> <font color="#000000">传统显微镜: Rxy=0.61l/NA</font><br /> <font color="#000000">Confocal: Rxy=0.4l/NA</font><br /> <font color="#000000">2. 样品的最大厚度:大约 2mm(10X/0.2物镜)</font><br /> <font color="#000000">取决于三点:</font><br /> <font color="#000000">物镜的景深, 与NA成反比</font><br /> <font color="#000000">Z轴的最大移动范围</font><br /> <font color="#000000">激光的穿透力</font><br /> <font color="#000000">3. 样品的最小光切厚度: 大约0.35 mm</font><br /> <font color="#000000">取决于两点: 物镜的数值孔径NA</font><br /> <font color="#000000">Z轴的最小移动步距: 40nm</font><br /> <font color="#000000">Z轴的分辨率: 0.35 mm</font><br /> <font color="#000000">4. </font><a href="http://www.lcgyjg.com/" target="_blank"><font color="#000000">激光</font></a><font color="#000000">器</font><br /> <font color="#000000">Kr/Ar激光器: 477nm, 488nm, 568nm, 647nm</font><br /> <font color="#000000">Ar激光器(UV): 361nm</font><br /> <font color="#000000">激光器的特点:</font><br /> <font color="#000000">1) 方向性好: 激光基本眼直线传播</font><br /> <font color="#000000">2) 单色性好 l=10-8nm</font><br /> <font color="#000000">3) 高亮度: 激光方向性好,其在空间上的能量分布是高度集中的</font><br /> <font color="#000000">4) 偏振性: </font><a href="http://www.lcgyjg.com/" target="_blank"><font color="#000000">激光</font></a><font color="#000000">为平面偏振光, 光纤耦合</font><br /> <font color="#000000">5. 四个荧光通道, 一个透射光通道, 即除了可同时采集</font><br /> <font color="#000000">多标记荧光图像外还可以同时采集透射光图像,但透</font><br /> <font color="#000000">射光图像为非共焦图像</font><br /> <br /> <font color="#000000">四. 共焦显微镜的类型：</font><br /> <font color="#000000">1.点(慢)扫描共焦显微镜: 分辨率高, 图像清晰;</font><br /> <font color="#000000">噪音低;</font><br /> <font color="#000000">采集图像速度慢;</font><br /> <font color="#000000">目镜中观察不到共焦图像;</font><br /> <font color="#000000">2.线(狭缝)扫描共焦 显微镜(video rate): 分辨率低;</font><br /> <font color="#000000">噪音高;</font><br /> <font color="#000000">扫描速度快 240幅/秒;</font><br /> <font color="#000000">目镜中可观察到共焦图像</font><br /> <font color="#000000">3.Nipkov转盘式扫描共焦显微镜: 性能介于上述两者之间</font><br /> <font color="#000000">功能:1.多荧光标记样品的图像采集</font><br /> <font color="#000000">2.无损伤、连续光学切片，显微“CT”</font><br /> <font color="#000000">3.真正的三维重组</font><br /> <font color="#000000">4.假三维图的显示</font><br /> <font color="#000000">5.可沿Z轴（xy平面）和Y轴（xz平面）方向进行光切</font><br /> <font color="#000000">6.定量分析</font><br /> <font color="#000000">7.xyt 、xzt、xyzt 和 xt 扫描―― 时间序列扫描</font><br /> <font color="#000000">8.图像处理</font><br /> <font color="#000000">9. 旋转扫描</font><br /> <font color="#000000">10.区域扫描</font><br /> <font color="#000000">11.光谱扫描</font><br /> <br /> <font color="#000000">五、显微CT与三维重组</font><br /> <font color="#000000">光切的层数：VoxelsizeZ &pound; 2VoxelsizeX</font><br /> <font color="#000000">七.动态<a href="http://opticaltest.oecr.com" target="_blank" class="relatedlink">测量</a> Physiology:细胞内离子动态变化测量</font><br /> <font color="#000000">1).游离Ca2+测量<a href="http://www.sdifk.com/" target="_blank"><font color="#f6e9ce"><font face="宋体"><strong>电动轿车配件</strong></font></font></a><a href="http://www.lcgyjg.com/" target="_blank"><font color="#f6e9ce"><font face="宋体"><strong>激光刻章机</strong></font></font></a><a href="http://www.gangguanhb.com/" target="_blank"><font color="#f6e9ce"><font face="宋体"><strong>无缝管</strong></font></font></a><a href="http://www.sdifk.com/" target="_blank"><font color="#f6e9ce"><font face="宋体"><strong>钨绞丝</strong></font></font></a><a href="http://www.252000.com/" target="_blank"><font color="#f6e9ce"><font face="宋体"><strong>聊城网络公司</strong></font></font></a><a href="http://www.lcgyjg.com/" target="_blank"><font color="#f6e9ce"><font face="宋体"><strong>激光雕刻机</strong></font></font></a></font><br /> <font color="#000000">检测游离Ca2+变化荧光探针的原理：这类探针多为Ca2+螯合剂，不与Ca2+结合时不发荧光，通常也不能进入细胞膜，只有与乙酰甲酯AM相连方可进入细胞内，被细胞内酯酶水解后并与胞内游离钙结合后发出荧光。Kd值为Ca2+解离常数，表明检测Ca2+浓度的范围:0.1xKd-10xkd,</font><br /> <font color="#000000">Kd和很多因素有关，如pH、 Mg2+、与蛋白的结合、温度等。细胞内生理Ca2+浓度值(10-100n</font><br /> <font color="#000000">M)，细胞内Ca2+超载浓度值（基础Ca2+浓度的10倍左右）</font></td></tr></table> </div> <div id="comment_514381" class="cm"> </div> <div id="post_rate_div_514381"></div> </div> </div> </td></tr> <tr><td class="plc plm"> <div id="p_btn" class="mtw mbm hm cl"> <a href="http://club.oecr.com/home.php?mod=spacecp&ac=favorite&type=thread&id=81242&formhash=95242a45" id="k_favorite" onclick="showWindow(this.id, this.href, 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style="color: #FF9900">chenchenchen</a></strong> <em>当前离线</em> </div><dl class="cl"> <dt>积分</dt><dd><a href="http://club.oecr.com/home.php?mod=space&uid=44196&do=profile" target="_blank" class="xi2">11547</a></dd> </dl><div class="imicn"> <a href="http://club.oecr.com/home.php?mod=space&uid=44196&do=profile" target="_blank" title="查看详细资料"><img src="static/image/common/userinfo.gif" alt="查看详细资料" /></a> <a href="http://club.oecr.com/home.php?mod=magic&mid=showip&idtype=user&id=chenchenchen" id="a_showip_li_514423" class="xi2" onclick="showWindow(this.id, this.href)"><img src="static//image/magic/showip.small.gif" alt="" /> IP卡</a> <a href="http://club.oecr.com/home.php?mod=magic&mid=checkonline&idtype=user&id=chenchenchen" id="a_repent_514423" class="xi2" onclick="showWindow(this.id, this.href)"><img src="static//image/magic/checkonline.small.gif" alt="" /> 狗仔卡</a> </div> <div id="avatarfeed"><span id="threadsortswait"></span></div> </div> </div> <div> <div class="avatar"><a href="http://club.oecr.com/space-uid-44196.html" class="avtm" target="_blank"><img src="http://www.oecr.com/uc_server/avatar.php?uid=44196&size=middle" /></a></div> </div> </div> </td> <td class="plc"> <div class="pi"> <strong> <a href="http://bbs.oecr.com/forum.php?mod=redirect&goto=findpost&ptid=81242&pid=514423" id="postnum514423" onclick="setCopy(this.href, '帖子地址复制成功');return false;"> <em>2</em>楼</a> </strong> <div class="pti"> <div class="pdbt"> </div> <div class="authi"> <img class="authicn vm" id="authicon514423" src="static/image/common/online_member.gif" /> <em id="authorposton514423">发表于 2009-1-18 17:17:49</em> <span class="pipe">|</span> <a href="http://bbs.oecr.com/forum.php?mod=viewthread&tid=81242&page=1&authorid=44196" rel="nofollow">只看该作者</a> </div> </div> </div><div class="pct"><div class="pcb"> <div class="t_fsz"> <table cellspacing="0" cellpadding="0"><tr><td class="t_f" id="postmessage_514423"> 路过,看了,谢谢!!!!!!</td></tr></table> </div> <div id="comment_514423" 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